Zimbabwean police have stopped several US and UK diplomats as they were trying to investigate political violence.
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He said their tyres were later slashed and a Zimbabwean driver working with a US security official was beaten up.
Last month, Mr McGee and diplomats from five other missions were briefly detained by Zimbabwean security forces.
Earlier, the South African government said Zimbabwe's main opposition leader, Morgan Tsvangirai, had been released by the police on Wednesday after the intervention of President Thabo Mbeki.
A spokesman for Mr Mbeki, Mukoni Ratshitanga, told the Associated Press that he "did engage the government and Mr Tsvangirai was released".
'Violation of protocols'
Mr McGee told the BBC that 10 US embassy officials and four officials from the UK High commission diplomats had been in Bindura, north of Harare, when they were stopped by the police.
When they refused to go to a local police station and drove away, they were chased, he said. Later, their tyres were slashed.
The ambassador said war veterans allied to the government had then threatened to set fire to the cars with the diplomats inside.
A Zimbabwean driver working with an US embassy security official was also beaten up by the group, he added.
Mr McGee described the incident as extremely serious and a violation of all diplomatic protocols, and that his government would raise it at the very highest levels with the Zimbabwean authorities.
The BBC's Caroline Hawley in Johannesburg says the incident comes as human rights groups talk of an escalating campaign of state-sponsored violence ahead of the presidential run-off vote between President Robert Mugabe and Mr Tsvangirai at the end of the month.
Mr Tsvangirai resumed his campaign after spending eight hours in detention, but there are growing fears about the credibility of the election, she says.
(BBC)
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